
Introduction to Lateral Flow Assays

● HOLICARE ACADEMY webinar - January 2025 ●
● HOLICARE ACADEMY webinar ●
23rd February 2024
● 7:00-8:30 AM EST ●
What to expect from this webinar ?
Lateral flow assays (LFAs) are widely used diagnostic tools known for their simplicity, speed, and versatility. This webinar will guide participants through the key stages of LFA development, from conceptualisation and design to optimisation and validation. Drawing on over two decades of expertise in rapid diagnostics, we will share insights into material selection, assay configuration, and troubleshooting common challenges. Whether you’re new to lateral flow development or seeking to refine your approach, this session offers practical strategies to enhance assay performance and reliability.
This webinar is presented by Michael Johnson from GADx, with Annelyse Duvoix present to answer questions.
Chapters:
00:04:58 Learning objectives
00:05:35 Introduction to Lateral Flow
00:07:09 How does a lateral flow assay work?
00:15:52 Antibodies – the cornerstone of LFAs
00:25:04 Design of LFAs
00:40:08 Validation and performance testing
● PROGRAM
- Understanding the origin and formats of bioinformatics data.
- Knowing the bioinformatics resources and the tools to acquire data for research.
- Understanding the local and global alignments and their applications
- Understanding the pipelines for genome assembly and variant discovery and their diagnostic applications with demonstration.
- Understanding the multiple sequence alignment and its application in phylogeny

HOLICARE Academy Expert

● Michael Johnson●
Michael Johnson is a Senior Scientist within the innovative R&D group at GADx. With over 20 years of experience in developing novel rapid diagnostics, including 11 years at GADx, he is dedicated to advancing diagnostic technologies. His expertise lies in creating platforms to evaluate and develop new antibodies for rapid diagnostic tests. He is also focused on designing supersensitive, low-cost, and robust assays to address critical health challenges in low- and middle-income countries, with a particular emphasis on Malaria and other neglected tropical diseases.
